Publications
Study Overview
This research is a part of my master’s thesis, investigating nuclear organization in cumulus cells across age groups in BALB/c mice. The project focuses on:
- 3D nuclear architecture: Quantifying spatial reorganization of nuclear bodies (e.g., Cajal bodies) during aging.
- Methodological innovation: Developed a novel modular macro for ImageJ/RStudio to:
- Automate nucleus segmentation.
- Quantify 3D parameters of nuclear bodies.
- Exclude artifacts.
Tool Description
The custom macro:
- Standardizes analysis: Reduces subjective errors in fluorescence microscopy data.
- Cross-adaptability: Compatible with other nuclear proteins (e.g., PML bodies).
- Open access: GitHub-hosted code with DOI:10.5281/zenodo.15395330.
Preliminary Findings
Application revealed:
- A threefold decrease in coilin-positive bodies in aged mice (12 mo.) vs. young (2–3 mo.).
- Age-dependent spatial reconfiguration relative to DAPI-dense regions.
Publication Timeline
Revised: 29 December 2025 / Accepted: 13 January 2026 / Published: 15 January 2026